Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/19
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We checked on the plates that were transformed yesterday (AubI and BjaI) and there were no colonies on the backbone or negative control plates using Ryan's NEB 10β cells! So I picked 3 colonies from each plate (shooting for ones that appeared red) and made 3mL liquid cultures. They are in the shaker. Colonies chosen are indicated on the plate.
I also fast-transformed the rest of the ligations (Bja, Cer, Esa, Las, Lux, Rpa, Sin) using NEB 10β cells. They are now in the incubator