Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/11/06

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  • Retried PCR of golden gate parts using Rene's primers, this time doing a Tm screen
Reagent uL
HF buffer10
primer F (10uM)2.5
primer R (10uM)2.5
dNTP (10mM)1
Template K0840070.5
Phusion polymerase 0.5
Total 50

  • annealed for 30 seconds
  • used Tm: 30, 32, 34, 36, 38
Number Template F Primer R Primer Annealing T Expected length
1K084007 3/10/13 4-2-1P069P07032645
2K084007 3/10/13 4-2-2P069P07032645

  • Still no clear bands observed for any of the annealing temperatures.

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