Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28

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PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20

Number Template F primer R primer Tm Expected length Expected incorrect length Product
1Receiver intP123P1124922032430Rec backbone
2 Receiver intP119P107481051pLux
3Receiver intP125P107481051pRhl
4Receiver intP126P107481051pEsa
5 F2620P113P11451817LuxR
6K092900+eCFP (70ul/ng)P117P11867765RhlR
7K092900+eCFP (74ng/ul)P117P11867765RhlR
8 K092900 (96ng/ul)P117P11867765RhlR
9EsaR synthesizedP121P12250758EsaR


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