Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/01

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04/01/2014

Sample Read# ng/┬ÁL fmol/ul ul Sample ul H2O
I13522 P111 P1238.17225.153846157.9510703362.048929664
G001 P105 P1075.05276.538461542.6130653277.386934673
Thing Volume (ul)
Part I1
Part II1
Part III1
T5 ligase1.25
BsmBI0.5
T4 DNA ligase buffer1
H2O4.25


GG reaction
Transformed into new DH5alphaT made by me soon after reaction finished. Long transformation, heat shock, 1.5 hour recovery time. New plates made by Behzad. Positive control plasmid, negative control of water.


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