miniprepped J06702 and the ligation colony I picked (backbone control plate had as many colonies as the ligation). Good yield for J06702, ok for other.
|J06702||0.247|| 0.128 ||1.93 ||247.2
|ligation||0.071|| 0.035 ||2.006||70.9
Digested J06702 with
- E only for 10 minutes
- X only for 10 minutes
- E and X for 10 minutes
- E only for 5, then add X for 5 more minutes
Digested ligation with
- P only
Ran on gel
Cut J06702 should be around 3kb. Possibly uncut, hence the crescent shapes and multiple bands.
Extracted the bottom bands from the two J06702 cut with E/X
Arrows show where the bands were extracted.
Zymo gel recover kit
|J06702 3||0.017 ||0.011 ||1.468|| 16.729
|J06702 4||0.008 ||0.005|| 1.583||7.782