Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/21

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

mm/dd/yyyy

Retry ligation from 10/19/13 again with gel extracted J06702
Backbone: J06702 E/X: 44 ng/ul

Cut, gel purified

Insert: Gblck pLac-RBS E/S: 2.6ng/ul

Cut, column purified

same calculations from before, still only actually tried 3:1 and bb ctrl

1:12:13:1Bb ctrl
insert0.51.01.50
vector1.131.131.131.13
2x Roche ligation buffer5555
T4 ligase1111
H202.371.871.372.87


ligations ran for 3 hours at RT
Incubate with 50ul DH5αT for 45 min on ice
heat shock for 30s at 42°C
ice for 2min
spread



|}


Personal tools