Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/18

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mm/dd/yyyy

Retry ligation with gel extracted J06702
Backbone: J06702 E/X: 44 ng/ul

Cut, gel purified

Insert: Gblck pLac-RBS E/S: 2.6ng/ul

Cut, column purified

did a bunch of calculations, only actually tried 3:1 and bb ctrl

1:12:13:1Bb ctrl
insert0.51.01.50
vector1.131.131.131.13
2x Roche ligation buffer5555
T4 ligase1111
H202.371.871.372.87


ligations ran for 25min at RT
Incubate with 40ul DH5αT for 15 min on ice



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