Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/28

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8am

Sender/ReceiverOD650GFPRFP
EsaI0.6791500
0.6711545
0.6341402
RhlI0.75513497
0.79214323
0.76313992
Ctrl0.7366645
0.7126485
0.7406598
LuxR-GFP0.5481619
0.5631858
0.5761957
LuxR-RFP0.576 768
0.564 682
0.562 694


Combined 3-5mL cultures of each sender into 15mL conicals. Spun in Wang lab large centrifuge
spun receiver cells in 2mL tubes at 12k g for 5min
I didn't write this down but I'm assuming I split the receiver cells into the sender supernatant in singlet.


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