Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/22

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J06702 mCherry 869bp -RBS-mCh-S-S-
Miniprepped F2620 and J06702 from 5/20/13, kept in fridge for about 24 hours. Yields seemed low so I picked more colonies. Possibly low yields from sitting in fridge. Will spin down and freeze in the future.

Part number 260 280 260/280 ng/ul
F2620 miniprep 1 0.018 0.012 1.535 18.413
F2620 miniprep 2 0.032 0.019 1.663 32.028
J06702 miniprep 1 0.009 0.006 1.367 8.633
J06702 miniprep 2 0.035 0.021 1.696 35.007


Picked two more colonies from F2620 and J06702 and the one colony on the F2620-E0240 ligation plate from 5/20/13


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