Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/23
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R-S didn't grow but R-only did. Picked R-colonies into S-R cultures. Added 1ml fresh LB in case the problem was old media, might need to check pH.
Used 100ul to 1ml of LB to test OD. Added 1ml H2), flicked tube, spun, poured off H2), added 100ul more.