Haynes Lab:Notebook/CRISPR Editing/2016/04/19
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Gal4-EED cells recently thawed and grown only in puro aren't growing well. I added new media with 20% FBS.
Maxiprep of g025, 31, 34 dCas9 plasmids. i froze bacterial pellets yesterday, thawed them and used the brafman lab macherey nagel maxi-prep kit