Haynes Lab:Notebook/CRISPR Editing/2016/02/05

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02/05/2016

Cass purified and ran sonicated DNA on gel
Lanes

  1. 1kb+ ladder
  2. Gal4 EED 1, unsonicated
  3. Gal4 EED 1, uncleaned and sonicated
  4. Gal4 EED 1, cleaned and sonicated
  5. Gal4 EED 2, unsonicated
  6. Gal4 EED 2, uncleaned and sonicated
  7. Gal4 EED 2, cleaned and sonicated
  8. Luc14 1, unsonicated
  9. Luc14 1, uncleaned and sonicated
  10. Luc14 1, cleaned and sonicated
  11. Luc14 2, unsonicated
  12. Luc14 2, uncleaned and sonicated
  13. Luc14 2, cleaned and sonicated
  14. 1kb+ ladder


Image:Screenshot 2016-03-12 20.37.14.png
Spec'd on nanodrop

Gal4 EED 1, cleaned and sonicated: 152.9 ng/uL
Gal4 EED 2, cleaned and sonicated: 204.7 ng/uL
Luc14 1, cleaned and sonicated: 99.2 ng/uL
Luc14 2, cleaned and sonicated: 122.2 ng/uL


Put samples at -20deg


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