Haynes Lab:Notebook/CRISPR Editing/2015/09/29

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

09/29/2015

isolated gDNA from puro dox g032 g046 cells from a few weeks ago. Set up PCR with P197/198

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 200ng 5ul - 200ng
Gal4-EEDpurog03210.2320.1152.012231.8550.864.14
Gal4-EEDpurog03220.2180.1082.02217.9540.924.08
Gal4-EEDpurog03230.1780.0882.008177.5131.133.87
Gal4-EEDdoxg03210.1040.0531.974104.2451.923.08
Gal4-EEDdoxg03220.0730.041.8472.9062.742.26
Gal4-EEDdoxg03230.0860.0432.00785.6162.342.66
Gal4-EEDpurog04610.2480.1241.994247.5060.814.19
Gal4-EEDpurog04620.3020.1492.027302.4680.664.34
Gal4-EEDpurog04630.2890.1461.985289.4250.694.31
Gal4-EEDdoxg04610.0670.0351.91166.5043.011.99
Gal4-EEDdoxg04620.1040.0541.937104.0221.923.08
Gal4-EEDdoxg04630.0740.0362.02373.8452.712.29

isolated gDNA from puro dox g054 g055 cells from a few weeks ago. Set up PCR with P197/198

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 200ng ul water to 10ul
Gal4-EEDpurog05410.0330.0142.28932.8796.083.92
Gal4-EEDpurog05420.0260.0122.23226.2947.612.39
Gal4-EEDpurog05430.0280.0132.25228.367.052.95
Gal4-EEDdoxg05410.0690.0332.10269.0232.907.10
Gal4-EEDdoxg05420.070.0342.09170.1962.857.15
Gal4-EEDdoxg05430.0610.032.05261.4513.256.75
Gal4-EEDpurog05510.1660.0822.033166.2481.208.80
Gal4-EEDpurog05520.2250.1141.981224.9640.899.11
Gal4-EEDpurog05530.190.0932.039190.4321.058.95
Gal4-EEDdoxg05510.10.052.021100.1832.008.00
Gal4-EEDdoxg05520.1150.0552.079114.6831.748.26
Gal4-EEDdoxg05530.1170.0572.07117.4611.708.30


Personal tools