Haynes Lab:Notebook/CRISPR Editing/2015/09/08

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

09/08/2015

Purified PCR products, set up annealing reactions for 24h time point

Cell type drug gRNA time post xfect rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Gal4doxg0342410.0760.0421.81376.2395.251.508.25
Gal4doxg0342420.0870.0481.81287.354.581.508.92
Gal4doxg0342430.080.0431.851805.001.508.50
Gal4purog0342410.0850.0481.78885.4254.681.508.82
Gal4purog0342420.050.0291.72550.1747.971.505.53
Gal4purog0342430.0780.0441.78377.7295.151.508.35


Ran nested PCR for the remaining samples for the time course experiment. 1:1000 dilution, P149/176, staggered the set up annealing reactions and surveyor for 48h and 72h, ran on BA:

Cell type drug gRNA time post xfect rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Gal4doxg0344810.0870.0491.78987.0494.601.508.90
Gal4doxg0344820.0890.0491.80888.6564.511.508.99
Gal4doxg0344830.080.0431.85379.6665.021.508.48
Gal4purog0344810.080.041.97279.7955.011.508.49
Gal4purog0344820.0860.0451.91185.8774.661.508.84
Gal4purog0344830.0690.0342.02468.8085.811.507.69
Cell type drug gRNA time post xfect rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Gal4purog0347210.0840.0441.9184.2744.751.508.75
Gal4purog0347220.0690.0361.9269.0155.801.507.70
Gal4purog0347230.0840.0441.92183.7484.781.508.72
Gal4doxg0347210.0630.0321.93762.926.361.507.14
Gal4doxg0347220.0590.0311.91959.3116.741.506.76
Gal4doxg0347230.0780.0411.88978.3415.111.508.39


Personal tools