05/14/2015
nested PCR with P163 and P215 of Gal4-EED cells, no dox, cut with g034. Dilutions of plasmid and lipofectamine LTX reagent in duplicate
| Cell type
|
Rep
|
ug plasmid
|
ul lipo
|
| Gal4-EED |
1 |
0.5 |
3
|
| Gal4-EED |
2 |
0.5 |
3
|
| Gal4-EED |
1 |
1 |
3
|
| Gal4-EED |
2 |
1 |
3
|
| Gal4-EED |
1 |
0.5 |
5
|
| Gal4-EED |
2 |
0.5 |
5
|
| Gal4-EED |
1 |
1 |
5
|
| Gal4-EED |
2 |
1 |
5
|
nested PCR with P163 and P215 of reopening chromatin experiment with KAH228. I already did the first replicate so I'm only doing the second replicate now
| 1
|
Luc14
|
GFP
|
g034
|
2
|
| 2 |
Luc14 |
GFP |
g034 |
3
|
| 3 |
Luc14 |
GFP |
KAH228 |
2
|
| 4 |
Luc14 |
GFP |
KAH228 |
3
|
| 5 |
Luc14 |
KAH228 |
g034 |
2
|
| 6 |
Luc14 |
KAH228 |
g034 |
3
|
| 7 |
Gal4-EED+dox |
GFP |
g034 |
2
|
| 8 |
Gal4-EED+dox |
GFP |
g034 |
3
|
| 9 |
Gal4-EED+dox |
GFP |
KAH228 |
2
|
| 10 |
Gal4-EED+dox |
GFP |
KAH228 |
3
|
| 11 |
Gal4-EED+dox |
KAH228 |
g034 |
2
|
| 12 |
Gal4-EED+dox |
KAH228 |
g034 |
3
|
| 
Project name
Main project page
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