Haynes Lab:Notebook/CRISPR Editing/2015/05/02

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

05/02/2015

Gal4-EED cells, +dox for 96 hours, -dox and transfected with either KAH228 or GFP, let grow for about 12 days, transfected with g025, g032, g046, or g048 on 4/29 at 6:30pm in triplicate.
Collected cells in lysis buffer, heat for 18mins at 56deg C, put at -20

Luciferase assay

' avg std dev
Luc142.750.08
KAH2281.020.06
GFP0.760.01


Personal tools