Haynes Lab:Notebook/CRISPR Editing/2015/01/23

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01/23/2015

Sent out untreated Luc14 gDNA PCR product of P197/P198 for sequencing:

Label Primer Direction
RD-1-3P175F
RD-4-6P161F
RD-7-9P173F
RD-10-12P159F
RD-13-15P170R
RD-16-18P156R
RD-19-21P172R
RD-22-24P158R



Quantification of editing efficiency with restriction enzyme'

cutting 1μg of untreated and g033 and g034 treated Luc14 cells gDNA PCRd with P197/P198 and column purified samples from 14.12.06 experiment with PfoI at 37°C for 3 hours.

Sample Read# ng/µL ul for 1ug ul H2O ul buffer ul PfoI
Untreated85.40211.715.2921
g03378.94212.674.3321
g03480.84112.374.6321

Quantification with 1% agarose gel







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