08/26/14
- CRISPR KAH154 cells - freeze stocks
- CRISPR KAH154 cells - dox-induce experimental and control samples
- KAH154 - expand to T-75 for new frozen stocks
CRISPR KAH154 cells - freeze stocks
- Harvested cells from 10 cm plates (100% confluent), 6 samples total
- CRISPR 1
- CRISPR 2
- CRISPR 4
- CRISPR 1d
- CRISPR 2d
- CRISPR 4d
- Resuspended pelleted cells in 5 mL freezing buffer, only used 2 mL of each (2 cryotubes) for frozen stocks
- Cells are at -80°C, to be tranferred to -150°C on Wednesday
CRISPR KAH154 cells - dox-inductions
- Induced the following samples (6-well plates, 100% confluent) with 1 μg/mL dox, except where noted
- CRISPR 1
- CRISPR 2
- CRISPR 4
- CRISPR 1d
- CRISPR 2d
- CRISPR 4d
- KAH154 (no dox) - RFP-negative ctrl.
- KAH154 +dox - RFP-positive ctrl.
- Also have extra plate with all CRISPR samples without dox
- Will do flow cytometry (tomorrow) on all samples on the Accuri machine for consistent data
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