Haynes Lab:Notebook/ASU iGEM/2012/06/26

6-26-12

• Transformation:

• Transformed DNA:

• double terminator (B0017, 2:6K)
• T7 RNA polymerase (I715038, 2:15C)
• puc19, negative control

• Protocol from: http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf (30 minute transformation)
• Cells: dh5a