Haynes Lab:Notebook/ASU iGEM/2012/06/19

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6-19-12 Transformation (LSE) Transformed DNA: T7 promoter BBa_I712074 Constitutive promoter BBa_J23102 Cells: DH5 alpha (donated) Protocol from: http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf (30 minute transformation) Controls: puc19, no DNA Plated 2 copies of each (100 ul, 250 ul) on LB amp plates. Made 50 LB Amp plates.