Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 3
MACS with strep and his plus cherry/red bacteria (07/02/07)
Run the MACS with the cherry/red bacteria incorporated to see if separation occurs (07/03/07)
- Followed Indirect Magnetic Labeling Protocol
- Used 300 ul of Strep/His and 300 ul of RFP Bacteria
- Note: OMPA1+Strep and OMPA1+His had low cell count
- Then follow Magnetic Separation
Results from MACS plate
Observation: Still have some background in the elution fraction, instead of the plain white strep and his colonies that we expected.
Conclusion: We may need to further optimize the wash steps, because some of the extraneous bacteria expected to be gone appear in the elution fraction.
Transformation of BL21DE3 RFP cells (07/05/07)
- For use in MACS assay.