Hartman Behavioral Neuroscience Lab:Notebook/Neonatal hypoxic-ischemia and hydrogen therapy/2008/05/09

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Neonatal hypoxic-ischemia and hydrogen therapy

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Animals weighed this afternoon
Conducted neurological evaluations using the "Garcia" (ref?) neurological evaluation for rodents (48-point scale):

Test	Scores
	0	1	2	3
1. Alertness	no response	response to pain	response to touch	response to sound (startle reflex)

Motor function:
2. Spontaneous activity (5 min)	No walking	Walks without wall approaching	Approaches 1-2 walls	Approaches 3-4 walls
3. Symmetry of walking	Falls while walking	Circling to one side	Turns to one side while pulling tail	Walking straight
4. Head/neck movement when suspended on tail	Turns <45 degree to both sides	Turns <45 degree to one side	Turns >45 degree to both sides	Turns >90 degree
5. Symmetry of forelimbs when suspended on tail	One front limb flexion	One front paw flexion	One front limb partial outreach	Symmetrical outreach
6. Climbing	Slides down within 2 seconds	Holds without climbing	Climbing with difficulty	Steady climbing
7.Balance on rod	Falls within 2 seconds	Holds without walking	Walking with difficulty	Steady walking

Sensory function:
8. Q-tip to Vibrissae	No head turning on both sides	No head turning on one side	Decreased response (one or both side)	Instant bilateral head turn
9. Q-tip to neck	No head turning on both sides	No head turning on one side	Decreased response (one or both side)	Instant bilateral head turn
10. Pin stick to body sides	No response on both side	One side no response	Decreased response (one or both side)	Instant bilateral response
11. Pinch to ears	No response on both side	One side no response	Decreased response (one or both side)	Instant bilateral response

Reflexes:
12. Corneal reflex	No blinks on both sides	No blink on one side	Decreased response	Instant bilateral blinks

1. tested for alertness (by response to touch (q-tip run down side of rat pup, either side); response to sound Researcher wanted to throw out (none were responding to sound (rub my fingers together by the ear). Response to pain (tail pinch); however researcher didn't want me to do anything to cause them pain. Motor Function Tests: 2. tested for spontaneous activity (for 5 min): in a cage top (in the Zhang lab) 0-3 score (no walking, walks without wall approaching, approaches 1-2 walls; approaches 3-4 walls 3. symmetry of walking: watched for falling, circling, or straight-walking 4. head/neck movement while suspended on tail: held rat by tail & watched for angle of head/neck movement. 5. symmetry of forelimbs when suspended on tail: watched for how they moved their limbs and forepaws. Occasionally touched rat down on hard surface to see how it reached with its paws 6. climbing (used the Zhang lab inclined plane): placed them in the center of the plane, was told to put them face up. Almost all of them climbed with some difficulty 8. balance on rod (used the Zhang lab neonate rod): placed rat with all limbs facing sideways on rod (as directed by an RA), allowed them to try to grasp, and then I let go. Watched for falling, walking, or just grasping Sensory function tests: 8. Q-tip to whiskers (held rat pup in hand and ran a q-tip end across whiskers (2x on one side, than switched to the other) 9. Q-tip to neck: ran q-tip down side of neck (2x) then switched to other side (measuring their head turn response) 10. we did not run pinstick to body (I was going to use the blunt end of the q-tip, but Researcher was adamantly opposed to this measurement, so I did not do it). 11. Again did not do pinch to ears; Researcher was opposed