Griffitts:Plasmids
From OpenWetWare
| Plasmid # | Parental plasmid | Marker | Notes |
|---|---|---|---|
| pJG245 | pJG210, pJG199 | NmR | In B083; promoterless lacZ |
| pJG246 | pJG210, pJG199 | NmR | In B083; same as pJG245 but without promoterless lacZ |
| pJG247 | None | NmR | pir-dependent; for E. coli loop-ins |
| pJG249 | pMAL-c2g | None | Perfect clone of pHISc2 |
| pJG250 | pJG181, pJG194 | None | GUS from pJG181 cloned into pJG194 |
| pJG255 | pHISc2 | ApR | feuPWT cloned with EcoR1/Xba |
| pJG256 | pMAC-c2g | ApR | feuPWT cloned with EcoR1/Xba |
| pJG259 | pRF771 | None | Gives lowest level of transcription from EcoR1 side |
| pJG263 | pJG259 | None | lacZ cloned into BamH1/Xba; Standard lacZ vector |
| pJG265 | pJG259 | None | GUS cloned into BamH1/Xba |
| pJG267 | pJG109 | TcR | TcR from pJG151 replaced NmR with EcoR1/BamH1 |
| pJG271 | pJG263 | TcR | PtauA::lacZ |
| pJG280 | pJG263 | TcR | mel promoter fragment (lacZ) cloned into EcoR1/Xba |
| pJG285 | pJG246 | TcR | In B083; TcR from pJG267 replaced KmR with EcoR1/BamH1 |
| pJG286 | pJG263 | None | PndvA cloned into EcoR1/Xba to make a lacZ fusion |
| pJG288 | pKD46, pJG109 | KmR | SC101TS from pKD46 and KmR-tnp from pJG109 were digested with AurII and ligated together |
| pJG289 | pKD46, pJG267 | TcR | SC101TS from pKD46 and tetA/R-tnp from pJG267 were digested with AurII and ligated together |
| pJG293 | pJG202, pJG271 | None | feuP from pJG202 cloned into Xba/BamH1 of pJG271; feuP expression should be taurine-inducible |
| pJG296 | pHISc2 | None | D51E mutation of feuP cloned with EcoR1/Xba |
| pJG297 | pHISc2 | None | D51A mutation of feuP cloned with EcoR1/Xba |
