Restriction Digest of Isolated GFP and ATF1 Plasmids
- Today we ran a restriction digest to double check the successfulness of our plasmid transformation. All ten samples were tested using a master mix of EcoR1, Pst1, DNA, and 10X Buffer. The positive control was plasmid to ensure the enzymes were working properly. The gel was run for 30 minutes and then an additional 15 minutes (to allow further movement of the bands of DNA). The results were excellent! Every sample had been successfully removed from its plasmid (two distinct bands were present in each column one representing the slightly smaller ATF1 and GFP bands and their larger vector bands). For Friday, Alex and I will finish designing our primers.