File:T7-1-sections alpha-beta.jpg

T7.1 genome design. (a) We split the wild-type T7 genome into six sections, alpha through zêta, using five restriction sites unique across the natural sequence. (b) Wild-type section alpha genetic elements: protein coding regions (blue), ribosome binding sites (purple), promoters (green), RNase III recognition sites (pink), a transcription terminator (yellow), and others (gray). Elements are labeled by convention (4). Images are not to scale, but overlapping boundaries indicate elements with shared sequence. The five useful natural restriction sites across section alpha are shown (black lines). (c) T7.1 section alpha parts. Parts are given integer numbers, 1 through 73, starting at the left end of the genome. Unique restriction site pairs bracket each part (red/blue lines, labeled D[part #]L/R). Added unique restriction sites (purple lines, U[part #]) and part length (# base pairs, open boxes) are shown. We do not know if sequence changes in and around parts 6 and 7 destroy the minor E. coli promoter, B. (d) Wild-type section beta genetic elements. (e) T7.1 section beta parts. Figure S1 in Appendix 2 depicts the six sections, alpha through zêta, which make up the T7.1 genome.