Endy:Screening plasmid/RBS library
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Can vary the Shine-Dalgarno (SD) sequence or the spacing between the SD and the start codon. If we're going to use oligo synthesis it will be easier to vary the SD to make a very large library. Alternatively we could make a small library with different spacing by simply synthesizing each one.
- Combine Heather's RBSs to make a library
- This might be an easy way to get a library of RBSs that are known to work (and in this case ready to be characterized as well).
- Synthesize an oligo library (would only vary the SD in this case, not the spacing).