DeLong:lLab Protocols:Julie's protocols
A warning: these all work for me, but molecular biology is a mysterious thing, so they may not work for you! Please email me at jmaresca (at) mit (dot) edu if you have a question or a problem. Thanks.
Preparation of electrocompetent cells. This works for E. coli, and I have not tested it on any other bacterial species. Image:Competent cells.pdf
Plasmid Minipreps. This is a standard miniprep protocol. Image:PlasmidMini.pdf
Standard PCR reactions (for normal amplification and for sequencing). Image:PCR jm.pdf
Use of the sonicator for small bacterial samples. Image:Delong Sonicator.pdf
Reverse-phase HPLC analysis of carotenoids and (bacterio)chlorophylls. Image:HPLC Tokyo.pdf
Reverse-phase HPLC analysis of carotenoids produced in E. coli. Image:HPLC Ecoli.pdf