Dandekar & Chandler:M9 Salts

Home        Contact        Internal        Protocols        Lab Members        Publications        Research        Talks       

10X M9 Salts (per Liter):

• Na2HPO4 60 g
• KH2PO4 30 g
• NaCl 5 g
• NH4Cl 10 g

Adjust pH to 7.4, autoclave

Before use at 1X add:

• 2.0 mL of sterile filtered 1M MgSO4
• 0.1 mL of sterile filtered 1M CaCl2