DIYbio:Notebook/Open Gel Box 2.0/Transilluminator
<html><a rel="license" href="http://creativecommons.org/licenses/by-sa/3.0/us/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-sa/3.0/us/88x31.png" /></a><br />This work is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by-sa/3.0/us/">Creative Commons Attribution-Share Alike 3.0 License</a></html>
Author List
Norman Wang <wangn(at)hawaii.edu>
Purpose
For fluorescing cyanine type DNA dyes, using off the shelf light source and excitation/emission filters. DIY units should be significantly cheaper depending on selection of parts, labor, and design.
Commercial Units
- E-Gel® Safe Imager™ Real-Time Transilluminator USD $545.00
- Safe Imager™ blue light transilluminator USD $1135.00
- Dark Reader transilluminators
- 14 x 21cm USD $695.00
- 30 x 46cm USD $1750.00
- 10 x 25cm USD $1250.00
DNA Dyes To Test
- Biotium
- GelGreen
- GelRed
- LabSupplyMall
- GR Safe
- GR Safe II
- Invitrogen
- SYBR Green
- SYBR Safe
- Lonza
- GelStar
- Any Company
- EtBR
Filter Wavelength Testing
Spectraphotometer readings of various Tiffen glass, colored acrylic pre and post light filters. Image under Creative Commons BY-SA 3.0 License
Empirical testing of filter for cyanine type DNA dyes. Performance of each filter can clearly be seen between #1-#2 and #3 orange emission filter. Image under Creative Commons BY-SA 3.0 License
However colored acrylic #2 has visible unwanted fluorescence along the edge. Image under Creative Commons BY-SA 3.0 License