Create running gel

From OpenWetWare

Jump to: navigation, search
To create 10ml of Running gel
Material 7.5% 9% 10% 11% 12.5%
30% Acrylamide 2.5 3 3.3 3.6 4.2
1.5M Tris pH8.8 2.5 2.5 2.5 2.5 2.5
10%SDS 100ul 100ul 100ul 100ul 100ul
10%APS 100ul 100ul 100ul 100ul 100ul
TEMED 10ul 10ul 10ul 10ul 10ul
Water 4.8 4.3 4 3.7 3.1
Personal tools