Combining Parts according to sequence homology
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Combinable Terminators
| Part number | Directionality/Efficiency | Lenght | Type | Location | Combine with |
| B0015 | Bi,98% (F), 30->60% (R) | 1 | Double | In stock | |
| B1001 | Bi,90% (F), 75% (R) | 1 | Single | ||
| B0014 | Bi,96% (F), 96% (R) | 1 | Double | In stock | |
| B0011 | Bi, ,95% (F), 86% (R) | 46bp | Plate 1, 2A | ||
| J61048] | Fwd 98% | 113bp |
Common vectors
| Plasmid | Replicon | Copy number |
| pBR322[1] and its derivatives | pMB1 | 15-20 |
| pUC vectors | pMB1 | 500-700 |
| pACYC and its derivatives | p15A[2] | 10-12 |
| pSC101 and its derivatives | pSC101[3] | 5 |
| ColE1 | ColE1[4, 5] | 15-20 |
Replicon compatibility
| Incompatibility group | Negative control element | Comment |
| colE1[4, 5], pMB1 | RNAI | controls processing of pre-RNAII into primer. pUC is derived from pBR322 (a single mutation in the pBR322 Primer RNA and deletion of the rop gene) which is derived from a pMB1 replicon, and cannot co-reside with the colE1 incompatibility group. |
| IncFII, pT181 | RNA | controls synthesis of RepA protein |
| P1, F, R6K, pSC101[3], p15A[2] | iterons | sequesters RepA protein |
Replication origins in different incompatibility groups are compatible. Replication origins in the same incompatibility group are not.
Some sets of vectors with compatible origins are available as a part of the Novagen Duet system. (from TK)
Individual vector links
Note: searching for cloning vector <insert vector name> when looking for vector sequences in NCBI Entrez Nucleotide search. It helps to cut down on the number of hits.
