Cconboy:Terminator Characterization

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Experiments performed by Caitlin Conboy, Fall 2003

Background

The rational design of integrated biological systems requires a set of characterized, compatible components. Here we characterize a set of transcriptional terminators contained in the registry of standard biological parts. [1]. We measure termination efficiency by inserting each terminator between the coding regions for CFP and YFP on a polycistronic message and measuring the fluorescence ratio of the two fluorophores by flow cytometry.

Constructs

Protocol

Results and Discussion

Related

Registry Wiki for Characterization of Parts

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Transcriptional Terminators in the Registry

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