Calendar:ImperialWetLabNotebook2009iGEM/2009-9-2

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--Dineka Khurmi 08:57, 2 September 2009 (EDT)

WEDSDAY JOB LIST

  • WaaL - Gel extraction - Run on gel with vector AK3 - Ligation
  • Harvard BB - Gel extraction - Run on gel with GFP - Ligation
  • CRP1 (vector) - Gel extraction - Run on gel with GFP - Ligation
  • Miniprep cellulase - Digestion - Run on a gel
  • Midiprep RFP I13507 (small culture)
  • Dirty ligation RBS + AK3 - Transformation
  • Pick culture colonies - grow 5ml
  • Make agar for Amp/Kan plates and LB media (send to autoclave)



David Roche 05:55, 2 September 2009 (EDT)
AM : Ran a gel with the WaaL pcr products that were digested overnight. 20ul against the ladder
Updated wiki and BioBrick registry


AM:

  • Checked transformed plates from yesterday - only OtsA and Ots B had colonies (1 or 2 each). Positive control worked indicating that the ligation was

responsible for poor results.

  • Did a mini Prep on the Edinburgh cellulases:

K118022

K118023

K118028

  • Went through and made corrections to SLIC primers for Construct 9.


PM:

  • Did Restriction digest on the Edinburgh cellulases and ran on gel. This indictated that the miniprep had been successful.
  • Plated OtsA and OtsB colonies from the Ak3+insert ligation plates (see AM for more details). Also kicked off a starter culture for a mini prep for these colonies which will be conducted tomorrow.
  • I transformed cells with the RBS + AK3 construct and plated out - will see results tomorrow following overnight incubation.
  • Innoculated media (200ml LB) for overnight incubation for tomorrow's midi on:

RBS-RFP-TT (BBa_I13507)

K118022

K118023

K118028

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