CAST:TolosaPublication

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Center for Advanced Sensor Technology

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LEAH TOLOSA, Ph.D.
Assistant Director
Center for Advanced Sensor Technology
University of Maryland Baltimore County
1000 Hilltop Circle, Baltimore, MD 21050
410-455-3432 (office), 410-455-6500 (fax)
email: leah@umbc.edu

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Journal Publications

  1. Bartolome, A., Smalls-Mantey, L., Lin, D., Rao, G., Tolosa, L. “FRET-based glucose monitoring for bioprocessing”, Genetically Engineered Probes for Biomedical Applications, Proc. of SPIE, 6098, 60980L-1 – 8, 2006.
  2. Kostov, Y., Smith, D. S., Tolosa, L., Rao, G., Gryczynski, I., Gryczynski, Z., Malicka, J., Lakowicz, J. R. (2005) Directional Surface Plasmon-Coupled Emission from a 3 nm Green Fluorescent Protein Monolayer, Biotech. Prog., 21, 1731-1735.
  3. Bartolome, A., Bardliving, C., Rao, G., Tolosa, L. (2005) Fatty acid sensor for low-cost lifetime-assisted ratiometric sensing using a fluorescent fatty acid binding protein, Anal. Biochem., 345, 133-139.
  4. Ge, X., Tolosa, L. (corresponding author), Rao, G. (2004) Dual-labeled glucose binding protein for ratiometric measurements of glucose, Anal. Chem., 76, 1403-1410.
  5. Ge, X., Tolosa, L., Simpson, J., Rao, G. (2003) Genetically engineered binding proteins as biosensors for fermentation and cell culture, Biotech. Bioeng., 84, 723-731.
  6. Tolosa, L., Ge, X., Kostov, Y., Lakowicz, J. R., Rao, G. (2003) Dual-emitting biosensors for glucose and glutamine from genetically engineered E. coli binding proteins, Proc. SPIE Genetically Engineered and Optical Probes for Biomedical Applications, 4967, 19-25.
  7. Kostov, Y., Tolosa, L., O’Connell, K., Anderson, P., Liu, Y., van Beek, N., Rao, G. (2003), Monitoring DsRed protein concentration in frozen insect larvae, Proc. SPIE Genetically Engineered and Optical Probes for Biomedical Applications, 4967, 100-107.
  8. Tolosa, L., Ge, X., Rao, G., (2003) Reagentless optical sensing of glutamine using a dual-emitting glutamine-binding protein, Anal. Biochem., 314, 199-205.
  9. Tolosa, L., Kostov, Y., Harms, P., Rao, G. (2002) Non-invasive measurement of dissolved oxygen in shake flasks, Biotech. Bioeng., 80, 594-597.
  10. Murtaza, Z., Tolosa, L., Harms, P. and Lakowicz, J. R. (2002) On the Possibility of Glucose Sensing Using Boronic Acid and Luminescent Ruthenium Metal-Ligand Complex, J. Fluorescence, 12(2), 187-192.
  11. Lakowicz, J.R., Gryczynski, I., Piszczek, G., Tolosa, L., Nair, R., Johnson, M.L., Nowaczyk, K. (2000) Microsecond dynamics of biological macromolecules, Methods in Enzymology, 23, 473-509.
  12. Kumar, C. V., Tolosa, L. M., (1999) Exploring the hydrophobic binding sites of calmodulin with fluorescent probes: Dynamic formation of the excimer, Indian Journal of Chemistry, 38B, 1170-1183.
  13. Lakowicz, J. R., Gryczynski, I., Gryczynski, Z., Tolosa, L., Dattelbaum, J. D., Castellano, F. N., Abugo, O. (1999) Emerging Fluorescence Technologies in Clinical Chemistry, Clinical Chemistry, Proceedings of the Oak Ridge Conference on the Road to Non-Invasive Testing.
  14. Lakowicz, J. R., Gryczynski, I., Dattelbaum, J.D., Tolosa, L., Rao, G. (1999) Novel Methods of Fluorescence Sensing, SPIE Conference on Advances in Fluorescence Sensing Technology IV, 3602, 234-240.
  15. Tolosa, L., Gryczynski, I. Randers-Eichhorn, L., Dattelbaum, J.D., Rao, G. and Lakowicz, J. R. (1999) Glucose Sensing, SPIE Conference on Advances in Fluorescence Sensing Technology IV, 3602, 46-51.
  16. Lakowicz, JR, Gryczynski I., Gryczynski Z., Tolosa, L. Dattelbaum, J. D.,and Rao, G. (1999) Polarization-Based Sensing with a Self-Referenced Sample, Applied Spectroscopy, 53(9), 1149-1157.
  17. Lakowicz, JR, Gryczynski I., Gryczynski Z., Tolosa, L. Randers-Eichorn, L.,and Rao, G. (1999) Polarization- Based Sensing of Glucose Using an Oriented Reference Film, Journal of Biomedical Optics, 4(4), 443-449.
  18. Tolosa, L., Gryczynski, I., Eichorn, L., Dattelbaum, J., Castellano, F. N., Rao, G. and Lakowicz, J. R.,(1999) Glucose Sensor for Low Cost Lifetime-Based Sensing Using a Genetically Engineered Protein, Anal. Biochem., 267(1), 114-120.
  19. Lakowicz, JR, Gryczynski, I., Tolosa, L., Dattelbaum, J. D., Castellano, F. N., Li, L. and Rao, G. (1999) Advances in Fluorescence Spectroscopy: Multi-Photon Excitation, Engineered Proteins, Modulation Sensing and Microsecond Rhenium Metal-Ligand Complexes, Proceedings of the Jablonski Conference, Acta Physica Polonica 99(1), 179-196.
  20. Lakowicz, JR, Castellano, F. N., Dattelbaum, J. D., Tolosa, L.,Rao, G.and Gryczynski, I (1998) Low Frequency Modulation Sensors Using Nanosecond Fluorophores, Anal. Chem., 70, 5115-5121.
  21. Tolosa, L. M., Malak, H., Rao, G. and Lakowicz, J. R., (1997) Optical Assay for Glucose Based on the Luminescence Decay Time of the Long Wavelength Dye Cy5TM , Sensors and Actuators B, 45, 93-99.
  22. Tolosa, L. M., Szmacinski, H., Rao, G. and Lakowicz, J. R. (1997) Lifetime-based Sensing of Glucose Using Energy Transfer with a Long Lifetime Donor, Analytical Biochemistry, 250, 102-108.
  23. Kumar, C. V. and Tolosa, L. M. (1994) 2,6-Bis(pyren-1-oyl)pyridine: A new Fluorescent Probe with a High Sensitivity to Hydrogen Bonding Solvents for the Development of Selective Sensors, J. Photochem. And Photobiol. A: Chem. Sec., 78, 63-69.
  24. Kumar, C. V. and Tolosa, L. M. (1993) Interaction of Hydrophobic Probes with Serum Albumin - Influence of the Side Chain and Exciplex Formation at the Binding Site, J. Phys. Chem., 97, 13914-13919.
  25. Tolosa, L. M. and Kumar, C. V. (1993) The Role of the Side Chain, Charge, and Hydrophobicity on the Protein Binding Affinity of Simple Fluorescent Molecules, FASEB J., 7(7), A1131.
  26. Kumar, C. V. and Tolosa, L. M. (1993) New Charge Transfer Probe for Solvent Polarity: Fluorescent Hydrogen Bonding Switch, Chem. Comm., 722.
  27. Radia, S. M., Kumar, C. V. and Tolosa, L. M. (1992) A New Approach for the Design of Sequence Specific Fluorescent Probes for DNA, Proceedings - National Conference for Undergraduate Research VI, 1796-1800.
  28. Tolosa, L. M. and Sevilla, F. B. (1987) Copper and Zinc in Hair Samples from Filipinos with Pulmonary Tuberculosis, Acta Manilana, 36, 51-57.


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