Plasmid Isolation and Liquid Media
Objectives:
- Analyze bacterial cultures inoculated 5/17
- Prepare LB Broth medium for the Synthetic Biology Lab
- Make a ampicillin stock for the SynBio Lab.
- Make overnight cultures
Bacteria Results
fas1b 2, KER 1D, Kn1 Recombined Plasmids in E.coli JM109
| Bacterial Strain | Growth (+/-) | Description
|
| JM 109 25μL
| + | Restreak (MC)
|
| JM 109 100μL
| +
| Good Growth (MC)
|
| Kn1 25μL
| + | Sm iso colonies
|
| Kn1 100μL
| + | MC to isolate
|
| KER 1D 25μL
| + | Sm iso colonies
|
| KER 1D 100μL
| + | MC to isolate
|
| fas1b 2 25μL
| + | iso colonies
|
| fas1b 2 100μL
| + | MC to isolate
|
MC= "multiple colonies present on agar"
LB Broth and Ampicillin Stock
-Made 300mL of LB broth media. Autoclaved at 9:47am
-Poured broth into 6, 50mL portions. Stored in SynBio Lab at room temp
-Started to make 10mL Amp (50mg/mL concentration) but spilled some during sterilization. Ended making 6mL of Amp stock and separating into 7 x's 700μL portions. Placed sterilized Amp in bullet box in Buckner freezer.
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|
Plasmids, Stocks
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