Images Taking and Data Analysis
1. Observed the slides under Laser scan Confocal Microscope(FluoViewTM FV1000, Olympus; excitation of Alexa 488).
2. For each cover slip, take 3 photos under different parts of the cover the slip.
3. Collect the photos and measure the signal intensity for each cell using software (Metamorph).
3. Collect data from Metamorph and use SPSS to calculate changes of intensity and the significance of the changes.