We used High-speed AFM(RIBM, Nano Live Vision).
silicon nitride cantilever:
resonant frequency = 1.0-2.0 MHz
spring constant = 0.1-0.3 N/m
EBDTip radius < 15 nm
resonant frequency = 1.5 MHz
spring constant = 0.1 N/m
EBDTip radius = 24nm
Scale bar : Figure.1-26, 200 nm
Old2D
This is a figure of 2D structure before cutting M13.
There is surplus M13(about 5000 bp) from the middle of the 2D structure since whole of M13 was used.
Buffer:1×TAE
Anealing time：2 hours
m13:staple＝1:100(ratio of concentration)
When Nickel was Put in,We observed AFM pictures clearly.
However, Nickel makes agregation increase while it makes adsorption power of mica heighten.
So we dicided not to use Nickel.
We confirmed M13:staple＝1:10(ratio of concentration)
In this concentration,it is easy to observe structures since agregation decrease.
Old3D
We can't observe the 3D structure using whole of M13.
As this factor,there are 3 points.
(1) The strucuture was not able to fold because of surplus M13.
(2) It was difficult to confirm the 3D structure in hispeed AFM.
(3) The 3D structure is similar to small agregation.
To observe the 3D structure,We need
(1)Few surplus M13(We succeeded after this experiment)
(2)Purification skill of agregation
Buffer:1×TAE
Anealing time：2 hours
m13:staple＝1:10(ratio of concentration)
