Biomod/2011/TUM/TNT/LabbookA/2011/10/27

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27th Oct 2011

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Contents

TEM

Prepared TEM Grids

According to standard procedures:

  • 2C9 & 2C10: BM2 control, 1:25 diluted with FOB20
  • 2D6: BM24 (15_65), 1:20 diluted with FOB20
  • 2D7: lost the grid before sample preparation
  • 2D8: BM24 (15_65), 1:10 diluted with FOB20
  • 2D9: BM24 (15_65), 1:10 diluted with FOB20

Observed Grids

  • 2A7 BM2 with 144nM DAPI: no origamis on the grid
  • 2C9 BM2 control: fine grid, 60 images
  • 2D6 BM24 control: good stain but low sample concentration, 24 images
  • 2D8 BM24 control: low sample concentration, 24 images
  • 2C9 BM2 control: good stain but to high dilution, 60 images
  • 2C10 BM2 control: fine grid, 60 images