Biomod/2011/TUM/TNT/LabbookA/2011/10/12

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12th Oct 2011

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Contents

TEM

Observed Grids

Observed grid 2B1: grid was fine.
Grids 2B2 and following (1:40 dilutions of BM2): dilution to high, very few particles
Decision: Prepare new grids with a 1:25 dilution

Prepared Grids: Biomod 2 D1, D2, D3

According to following scheme: pipet plan
D1: One ethidium bromide should bind in average every 7bp
D2: One ethidium bromide should bind in average every 21bp
D3: Control without any DNA binders

Observation

Again only few particles.
For these grids, a new purification of BM2 was used, perhaps this purification had a smaller yield than the last one, resulting in smaller concentrations here.
Besides that, staining and particles is fine.