Biomod/2011/TUM/TNT/LabbookA/2011/09/20

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20th Sep 2011

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Measured Concentrations of labeled Oligonucleotides

The concentrations of the oligonucleotides were determined according to absorption at 260 nm.

The U structure labeled with Atto 550 ddCTP:

  • the_U_172, helix 4, c = 10.1 µM
  • the_U_175, helix 4, c = 5.7 µM
  • the_U_183, helix 5, c = 2.8 µM
  • the_U_197, helix 8, c = 11.1 µM

The U structure labeled with Atto 647N ddUTP:

  • the_U_173, helix 4, c = 9.9 µM
  • the_U_204, helix 20, c = 8.2 µM
  • the_U_218, helix 23, c = 7.1 µM
  • the_U_225, helix 29, c = 6.0 µM