Biomod/2011/TUM/TNT/LabbookA/2011/09/19

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19th Sep 2011

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Contents

Labeled Oligonucleotides

According to standard protocol
labeled with Atto 550 ddCTP at the following positions (staple, helix):

  • the_U_172, helix 4
  • the_U_175, helix 4
  • the_U_183, helix 5
  • the_U_197, helix 8

labeled with Atto 647N ddUTP at the following positions (staple, helix):

  • the_U_173, helix 4
  • the_U_204, helix 20
  • the_U_218, helix 23
  • the_U_225, helix 29



Determination of Absorption Coefficient of unlabeled the_U_172 and the_U_173

A260 (the_U_172) = 2.99 x 105 M-1 cm-1
A260 (the_U_173) = 2.87 x 105 M-1 cm-1