Biomod/2011/TUM/TNT/LabbookA/2011/09/19

Labeled Oligonucleotides

According to standard protocol
labeled with Atto 550 ddCTP at the following positions (staple, helix):

• the_U_172, helix 4
• the_U_175, helix 4
• the_U_183, helix 5
• the_U_197, helix 8

labeled with Atto 647N ddUTP at the following positions (staple, helix):

• the_U_173, helix 4
• the_U_204, helix 20
• the_U_218, helix 23
• the_U_225, helix 29

Determination of Absorption Coefficient of unlabeled the_U_172 and the_U_173

A₂₆₀ (the_U_172) = 2.99 x 10⁵ M^-1 cm^-1
A₂₆₀ (the_U_173) = 2.87 x 10⁵ M^-1 cm^-1