Biomod/2011/TUM/TNT/LabbookA/2011/09/15

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15th Sep 2011

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Contents

Fluorescence Microscope bulk Measurements with Spermine

Recording of 10 films each 50 frames at 50 ms exposure time each picture for:

  • BM5_5/20 without DNA-binder
  • BM5_5/20 with 100 µM spermine
  • BM5_5/20 with 10 µM spermine


  • BM4_8/23 without DNA-binder
  • BM4_8/23 with 100 µM spermine
  • BM4_8/23 with 10 µM spermine


  • BM3_4/29 without DNA-binder
  • BM3_4/29 with 100 µM spermine
  • BM3_4/29 with 10 µM spermine


Results

Samples with 100 µM spermine displayed many bright spots (figure 1), we should check at the TEM for aggregates.


Figure 1:
Structure BM_4/29, 100 µM spermine, green laser


Figure 2:
Structure BM_8/23, 100 µM spermine, green laser


Figure 3:
Structure BM_5/20, 100 µM spermine, green laser