Biomod/2011/TUM/TNT/LabbookA/2011/08/17

Measuring the Absorption Coefficients and Concentrations of Single Stranded Labled DNA

determined the absorption coefficients of MH_255 and MH_256 as well as the concentrations of the labeled MH_255 (Atto 647N ddUTP) and MH_256 (Atto 550ddCTP) according to standard procedures

Results

ε₂₆₀ (MH_255) = 1.58e5 M^-1cm^-1
ε₂₆₀ (MH_256) = 1.59e5 M^-1cm^-1
coefficients are in good agreement with structure based prediction

c (MH_255 Atto 647N ddUTP) = 2.8µM
c (MH_256 Atto 550 ddCTP) = 7.1µM