- This morning I spent a lot of time working on the Project section of our wiki. Check it out at HarvarDNAnos Project Page!
- I also investigated the activity of the Restriction Enzyme NOT1 and began designing a mechanism of incorporating its recognition sequence into the seam of the sphere in order to facilitate opening of the sphere. In specific, I designed a construct to test whether or not a restriction enzyme motif would be sufficient to open our sphere. Note in the image below, if the hairpin is open we should be able to detect this on a gel, and this will show that a given amount of pre-cut excess is sufficient to allow a R.E. to cut but sphere to open.
- Testing NOT1 Restriction Enzyme
- Our Week 2 Team Photo!