BioSysBio:abstracts/2007/Najl Valeyev

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Mathematical Modelling of Multi-site Protein-Ligand Interactions

Author(s): Najl V Valeyev1,2, Nikolai V Kotov2, Ian Postlethwaite1, Declan Bates1
Affiliations: 1 Control and Instrumentation Research Group, Department of Engineering, University of Leicester, University Road, Leicester, LE1 7RH, UK, 2 Biophysics & Bionics Lab, Department of Physics, Kazan State University, Kazan, Russia, 420008 Contact: email: nv28@le.ac.uk
Keywords: multisite proteins', 'Hill equation', 'multifunctional regulation', 'dose-response curve', 'IC50', 'EC50'

Background/Introduction

Protein molecules with multiple binding sites play a fundamental role in regulation of a large number of ferments, ion channels and other cellular components. Mathematical modelling of multisite protein-ligand interactions which should be based on realistic assumptions is very important for understanding complex biological networks.

Images

Figure 1. Comparison of multisite protein ligand binding models     The dose-response curves are shown for a protein with four binding sites using following kinetic schemes: a) the Hill equation; b) the consecutive binding; c) the independent binding. The same values have been used for all three models.
Figure 1. Comparison of multisite protein ligand binding models

The dose-response curves are shown for a protein with four binding sites using following kinetic schemes: a) the Hill equation; b) the consecutive binding; c) the independent binding. The same values have been used for all three models.

Materials/Methods

Frequently the experimental dose–response curves for multisite protein interactions with ligand are analysed using the Hill equation [Hill, 1910]. Although the Hill equation is widely used in biological pathways modelling as well as in pharmacodynamic studies [Aviles et al., 2000], it is derived based on a physically unrealistic assumption according to which all ligand molecules simultaneously bind to a protein. In effect, this assumption is only valid in the case of extreme positive cooperativity.

Results

We developed models for physically realistic consecutive and independent binding schemes and compared them with the Hill equation (Figure 1). It follows from this comparison that the “half-effect” concentration may significantly differ from the conventional Hill equation depending on the binding mechanism.

We used our models to rigorously investigate mechanisms for second messenger activation by multisite proteins. We show that differential and selective activation of different targets by the same protein-ligand complex (Ca2+ and calmodulin, for example) can be achieved by biologically active non-saturated intermediate multisite protein-ligand complexes.


References

Aviles, P., Aliouat, E. M., Martinez, A., Dei-Cas, E., Herreros, E., Dujardin, L. & Gargallo-Viola, D. (2000). In vitro pharmacodynamic parameters of sordarin derivatives in comparison with those of marketed compounds against Pneumocystis carinii isolated from rats. Antimicrob Agents Chemother 44, 1284-90.
Hill, A. (1910). The combinations of haemoglobin with oxygen and with carbon monoxide. J Physiology 40, 4-7.

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