Berglund:PCR primers for splicing

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T7primer: 5'TAA TAC GAC TCA CTA TAG GG


pcDNA3 F1: 5'ATT AAT ACG ACT CAC TAT AGG GAG ACC C, PCR Cycles: 24, Elongation Temp. (C): 55
pcDNA3 R1-RT Primer: 5’ AGC ATT TAG GTG ACA CTA TAG AAT AGG G, PCR Cycles: 24, Elongation Temp. (C): 55
TNNT2 F1 (cTNT): 5'-GTT CAC AAC CAT CTA AAG CAA GAT G, PCR Cycles: 24, Elongation Temp. (C): 55
TNNT2 R1 (cTNT): 5'-GTT GCA TGG CTG GTG CAG G, PCR Cycles: 24, Elongation Temp. (C): 55
MBNL1 F1 (short): 5'-GAT CAA GGC TGC CCA ATA CCA G, PCR Cycles: 26, Elongation Temp. (C): 55
MBNL1 R1 (JP): 5'-CAG ATT CAT TTA TTA AGA AAC CCC ACC CCT TAC, PCR Cycles: 26, Elongation Temp. (C): 55
Serca F1-RT Primer (Stacey): 5'-ACC TCA CCC AGT GGC TCA TG
Serca R1-RT Primer (Stacey): 5'-CCA CAG CTC TGC CTG AAG ATG TG
RTlacSERCA1-e21 F1: 5'-GTC CTC AAG ATC TCA CTG CCA GT, PCR Cycles: 20, Elongation Temp. (C): 55
RTlacSERCA1-e23 R1: 5'-GCC ACA GCT CTG CCT GAA GAT G, PCR Cycles: 20, Elongation Temp. (C): 55
INSR RT Primer (vector specific): 5'-TCG TGG GCA CGC TGG TCG AG, PCR Cycles: 22, Elongation Temp. (C): 55
INSR F1 (DM1-splice): 5'-CCA AAG ACA GAC TCT CAG AT, PCR Cycles: 22, Elongation Temp. (C): 55
INSR R1 (DM1-splice): 5'-AAC ATC GCC AAG GGA CCT GC, PCR Cycles: 22, Elongation Temp. (C): 55
ATP2A1 F1 (DM1-splice): 5'-TTC GTT GCT CGG AAC TAC C, PCR Cycles: 20, Elongation Temp. (C): 55
ATP2A1 R1 (DM1-splice): 5'-GGT TGG GAA GGG GAA TTT AC, PCR Cycles: 20, Elongation Temp. (C): 55
DUP RT Primer and R1: 5'-GCA GCT CAC TCA GTG TGG CA, PCR Cycles: 24, Elongation Temp. (C): 58
DUP F1: 5'-GAC ACC ATG CAT GGT GCA CC, PCR Cycles: 24, Elongation Temp. (C): 58
pcDNA3-R1 (RT Primer PLEKHH2-SM1F): 5'- AGC ATT TAG GTG ACA CTA TAG AAT AGG G, PCR Cycles: 23, Elongation Temp. (C): 58
PLEKHH2-SM1 F1: 5'-GCT GCA GAA TGA AAT TTG CTG TCA G, PCR Cycles: 23, Elongation Temp. (C): 58
PLEKHH2-SM1 R1: 5'-GTC ACC ATT TTG TTG CGT TCT TTC TAC, PCR Cycles: 23, Elongation Temp. (C): 58
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