Berglund:PCR Buffer

10x PCR Buffer (LabTaq)

• 6.25ml 4M KCl (fc=500mM)
• 5ml 1M Tris pH 8.9 (fc=100mM)
• 0.5ml Triton-x-100 (fc=1%)
• 0.75ml 1M MgCl₂ (fc=15mM)
• 37.5ml water

6x DNA dye (for splicing gels)

• 100µl 1M Tris pH7.5 (fc=1.7mM)
• 4ml 100% glycerol (fc=6.7%)
• 2.4ml 0.5M EDTA pH 8 (fc=20mM)
• 12mg bromophenol blue (fc=0.3mM)
• 15mg xylene cyanol (fc=0.5mM)
• water to 10ml

Sybr Green dye

• 1.5ml 6x DNA dye
• 1.2µl 10,000 SYBRI dye