Berglund:PCR Buffer

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10x PCR Buffer (LabTaq)

  • 6.25ml 4M KCl (fc=500mM)
  • 5ml 1M Tris pH 8.9 (fc=100mM)
  • 0.5ml Triton-x-100 (fc=1%)
  • 0.75ml 1M MgCl2 (fc=15mM)
  • 37.5ml water


6x DNA dye (for splicing gels)

  • 100µl 1M Tris pH7.5 (fc=1.7mM)
  • 4ml 100% glycerol (fc=6.7%)
  • 2.4ml 0.5M EDTA pH 8 (fc=20mM)
  • 12mg bromophenol blue (fc=0.3mM)
  • 15mg xylene cyanol (fc=0.5mM)
  • water to 10ml


Sybr Green dye

  • 1.5ml 6x DNA dye
  • 1.2µl 10,000 SYBRI dye
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