Berglund:Materials

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10x yeast pyrophosphatase

50% glycerol
10mM HEPES-KOH pH 7.5
6mM MgCl2

120µg yeast pyrophosphatase from Sigma i1891 (0.01U/µl)= 120U lyophilized and would be diluted into a fv=12ml
But, you may receive less than 120µg and so you dilute accordingly (100µg =100U= fv of 10ml for 10x stock)

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