BME100 s2016:Group10 W1030AM L1
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LAB 1 WRITE-UP
Independent and Dependent Variables
The independent variable is the dosage of lipopolysaccharide (in mg). This is the independent variable because the dosage of lipopolysaccharide is what is being manipulated.
Number of subjects per group
The subjects will be randomly assigned to the six groups with 10 subjects in each group. Each patient group will undergo ELISA testing to determine their initial protein level. After the initial protein level measurement, patients will receive their designated dosage of lipopolysaccharide. Once the dosage has been administered, patients will undergo a second ELISA screening to measure protein levels. After the results are gathered from all five groups, the change from initial to final inflammotin levels will be recorded. These results will be analyzed to determine the lowest dosage of lipopolysaccharide that causes an increase of inflammotin protein in the elderly.
Our subjects will be in the age range 65-75 (senior citizens). We will post flyers and ads for our study in senior centers in the surrounding areas of Tempe (such as communities like Sun Lakes) describing our experiment and parameters. The subjects will be volunteers and will need to complete a preliminary health screening to ensure that the subjects do not have a pre-existing condition or anomaly that will affect the baseline inflammotin protein. Those who show up for the experiment will be added to a pool. From there, subjects who meet the health requirements will be randomly assigned to one of the five groups based on a random number generator. Although subjects who volunteer for the study will be volunteering for a specific reason, we cannot allow completely random patients to participate without consent. Becayse of this, there will be patient bias in the study, but a bias that we will take into account. The patients in the 0 mg group will be given a placebo pill and their inflammotin protein levels will be used as the control.
Sources of Error and Bias
Potential sources of error or bias can include: The volunteers may already have existing conditions that would cause higher levels of the inflammotin protein. This can cause inaccurate data when measuring protein levels. We would control for these errors by having the preliminary health screening to ensure that patients do not have abnormal levels of inflammotin.