BME100 s2014:W Group14 L2

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
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Contents

OUR TEAM

Name: Jose Duran
Name: Jose Duran
Name: Shawn Garcia
Name: Shawn Garcia
Name: Christian Keefer
Name: Christian Keefer
Name: Karthik Puncha
Name: Karthik Puncha
Name: Austin Tielke
Name: Austin Tielke

LAB 2 WRITE-UP

Descriptive Statistics

Experiment 1

Human Study

LPS Dose Average Standard Deviation Endpoint Standard Error
0 mg 3.834 1.523010177 10 0.48161811
5 mg 8.932 1.593931547 10 0.50404541
10 mg 61.622 30.11069386 10 9.52183745
15 mg 657.941 212.9429762 10 67.3384817



Experiment 2

Rat Study

LPS Dose Average Standard Deviation Endpoint Standard Error
0 mg 10.516 2.225551617 5 0.995296941
10 mg 11.112 7.402885924 5 3.310671231



Results

Experiment 1


Experiment 2



Analysis

Experiment 1

ANOVA Test: Single Factor
SUMMARY

Groups Count Average Variance
0 mg 10 3.834 2.31956
5 mg 10 8.932 2.540618
10 mg 10 61.622 906.6539
15 mg 10 657.941 45344.71
Source of Variation SS dF MS F p-level F crit
Between Groups 3027016.69 3 1009005.56 87.2536019 1.40E-16 2.86626555
Within Groups 416306.027 36 11564.0563
Total3443322.7239


Post-hoc Testst-test value Adjusted P-value to achieve significance Significant?
0mg vs. 5mg 8.59631E-07 0.008333333 Yes
0mg vs. 10mg 9.94377E-06 0.008333333 Yes
0mg vs. 15mg 1.39436E-08 0.008333333 Yes
5mg vs. 10mg 3.01859E-05 0.008333333 Yes
5mg vs. 15mg 1.57101E-08 0.008333333 Yes
10mg vs. 15mg 6.4824E-08 0.008333333 Yes


Experiment 2

t-test value P-value to achieve significance Significant?
0.867403497 0.05 No



Summary/Discussion


With regards to the first experiment, the results showed that an increased dosage of lipopolysaccharide (LPS) increased the levels of inflammotin proteins found in the body. The Bonferroni Correction indicated that the smallest dosage (5 mg) resulted in an increase in inflammotin protein levels which was proved by the significant difference between the 0 mg to 5 mg dosages. Although there was a high variance among the test subjects who took higher doses, each increasing dosage of LPS resulted in statistical significance.

Regarding the second experiment performed on rat subjects, 0mg and 10mg doses were given to a two groups of five rat subjects. It was found that there was no significant difference calculated upon the application of an unpaired t-test because the t-test value (0.86) was greater than the 0.05 p-value.

When analyzing the data between the two experiments, it can be stated that there is no significant impact of LPS on the rat subjects; however, there was a significant difference between the groups of the human subjects. It can be inferred that the human body is more sensitive to LPS than the body of a rat. Future experiments could be performed that involve a higher dosage of LPS given to rats if justification is needed to prove whether or not the body of a rat is significantly sensitive to the chemical compound lipopolysaccharide.

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